Anti-PEG Antibody
ANPEG-1 mouse monoclonal IgM against PEG has been successfully demonstrated as a useful tool not only in immunogenicity assays, but also in pharmacokinetic (PK) assays for the detection of PEG or intact PEGylated drugs.
It can be used as the capture or detector antibody in a PK sandwich ELISA. In Figure 1, ANPEG-1 is used as a capture antibody directly coated onto a regular 96 well plate (Precoated ANPEG-1 plates, P/N 11-5001). In this PK ELISA, the resulting sandwich complex will consist of ANPEG-1 capture antibody attaching the PEGylated protein drug via its PEG chain, and an enzyme-labeled detector antibody against the protein moiety of the PEGylated drug.
Figure 2 is an example of ANPEG-1 used as the detector antibody in a PK sandwich ELISA for PEGylated protein drugs. It can be conjugated directly to an enzyme such as horseradish peroxidase (HRP) and be combined with a capture antibody specific to the protein moiety of a PEGylated protein drug.
Alternatively, the ANPEG-1 detector can be biotinylated and a streptavidin-HRP conjugate can be used to label in situ the ANPEG-1 detector in sandwich complexes formed in a PK sandwich ELISA as shown in Figure 3.
| Product No. | Description | Price* |
| 90-1010-100UG | Anti-PEG monoclonal IgM Antibody (ANPEG-1) (100 µg) | $249.00 |
| 90-1061-100UG | Anti-PEG Monoclonal IgM Ab without stabilizer (ANPEG-1) (100ug) | $249.00 |
| 90-1052-100UG | Biotinylated Anti-PEG monoclonal IgM Antibody (ANPEG-1) (100 µg) | $350.00 |
| 11-5001-10PK | NIDS® HyperBind anti-PEG Ab-precoated clear solid ELISA plates (10 Plates) | $1,250.00 |
| High Sensitivity Plates | ||
| 11-1001-5PK | NIDS® HyperBind High Sensitivity Streptavidin Coated Clear Solid Plates (96 Well) (5 Plates) | $125.00 |
| 11-1007-5PK | NIDS® HyperBind High Sensitivity Neutravidin Coated Clear Solid Plates (96 Well) (5 Plates) | $125.00 |
| 12-1001-5PK | NIDS® HyperBind High Sensitivity Streptavidin Coated Black Solid Plates (96 Well) (5 Plates) | $125.00 |
| 13-1001-5PK | NIDS® HyperBind High Sensitivity Streptavidin Coated White Solid Plates (96 Well) (5 Plates) | $125.00 |
| High Binding Plates | ||
| 11-3001-5PK | NIDS® HyperBind High Binding Streptavidin Coated Clear Solid Plates (96 Well) (5 Plates) | $125.00 |
| 11-3007-5PK | NIDS® HyperBind High Binding Neutravidin Coated Clear Solid Plates (96 Well) (5 Plates) | $125.00 |
*Bulk discounts available
Please click JPTM paper for additional information on the use of ANPEG-1 anti-PEG monoclonal IgM Antibody.
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